SP 1: Optimized provision of standardized and characterized mouse and human hepatocytes for and technical assistance within the network HepatoSys |
Prof. Rolf Gebhardt, Leipzig |
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SP 2: Primary mouse hepatocytes (SOP´s) and comparison of cultured mouse hepatocytes to the intact liver |
Prof. Jan Hengstler, Dortmund |
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SP 3: Isolation of human hepatocytes for the Competence Network HepatoSys |
Prof. Andreas Nüssler and PD Dr. Natascha Nüssler, Berlin |
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SP 4:Transfection and siRNA mediated gene knockdown SOP´s |
Prof. Steven Dooley, Mannheim |
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SP 5: Transduction and siRNA mediated gene knockdown SOP´s |
Prof. Marino Zerial, Dresden |
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SP 6: Engineering methods for defined short term exposure of hepatocytes cultured according to SOP, SOP proliferating hepatocytes and quantification of cell proliferation and scattering |
Prof. Dr.-Ing. Klaus Affeld and Dr.-Ing. Ulrich Kertzscher |
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SP 7: Stabilization of hepatocyte cultures by defined extracellular matrix |
Dr. Brigitte Angres, Tübingen |
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SP 8: Characterization of murine hepatocyte cultures vs. intact mouse liver |
Dr. Freimut Schliess, Dr. Johannes Bode and Prof. Diester Häussinger, Düsseldorf |
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SP 9: Isolation of human hepatocytes for the Competence Network HepatoSys |
Dr. Wolfgang Erwin Thasler, München and Dr. Thomas Weiss, Regensburg |



